Sialylated Mucin-Type-O-Glycans antibody

Catalog No. ABIN6757949

This anti- antibody is a Mouse Monoclonal antibody detecting in IHC (p), ELISA, IHC and ICC. Suitable for Human.

Quick Overview for Sialylated Mucin-Type-O-Glycans antibody (ABIN6757949)

Target: Sialylated Mucin-Type-O-Glycans

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: FW17

Product Details

Specificity: Sialylated Mucin-Type-O-Glycans.

Purification: Purified

Immunogen: Purified mucus-glycoproteins from human amniotic fluid.

Isotype: IgM

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P

Usage: Tumor and normal tissues were first fixed in 6 % neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 μm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2 % H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5 % in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2 % in PBS), (4) swine anti-rabbit immunoglobulin (2 % in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/mL - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01 % H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21 °C for 30 min.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details

Target: Sialylated Mucin-Type-O-Glycans